Journal: Journal of Inflammation (London, England)
Article Title: Cannabinoid 2 receptor attenuates inflammation during skin wound healing by inhibiting M1 macrophages rather than activating M2 macrophages
doi: 10.1186/s12950-018-0201-z
Figure Lengend Snippet: a and b Representative immunoblotting results and ( c ) semi-quantitative analysis of CB2R protein level at different posttraumatic intervals. + P < 0.05, ++ P < 0.01, JWH133 versus vehicle; * P < 0.05, ** P < 0.01, GP1a versus vehicle; # P < 0.05, ## P < 0.01, AM630 versus vehicle
Article Snippet: After being blocked with 5% fat-free milk at RT for 2 h, PVDF membranes were incubated, respectively, with a rabbit anti-CB2R polyclonal primary antibody (1:250 dilution; sc-25,494, Santa Cruz Biotechnology, CA, USA) and mouse anti–GAPDH monoclonal antibody (1:2500 dilution, sc-32,233; Santa Cruz Biotechnology, Inc) at 4 °C overnight.
Techniques: Western Blot